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Updated: Jun 28, 2026

A Study of the Complexation of Mercury(II) with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry
Published on: January 8, 2016
A low consumption air-segmented sequential injection vapor generation system for the determination of mercury by
1Research Center for Analytical Sciences, Box 332, Northeastern University, Shenyang, 110006, China.
Abstract:
A sequential injection system for the determination of mercury by vapor generation atomic absorption spectrometry (VGAAS) using tetrahydoborate reductant was developed, characterized by prevention of sample and reagent mixing in the holding coil using small air segments and initiation of the vapor generation in a flow-through gas-liquid separator. Extremely small volumes of reductant of 15-30 mul (0.2-1.0% NaBH(4)) and sample acidity as low as 0.05 mol l(-1) HCl were sufficient for achieving performance similar to flow injection (FI) VGAAS systems. A sample throughput of 90 h(-1) was achieved with 400 mul samples with a precision of 2.0% RSD at 10 mug l(-1)Hg, and a detection limit of 0.1 mug l(-1) (3sigma). Reagent consumption was reduced by a factor of 25 in comparison to the FI-VGAAS system. Good agreement with the certified value was obtained for the determination of mercury in seawater in a standard reference sample.
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