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Published on: March 31, 2019
Fluorescence energy transfer between Acridine Orange and Safranine T and its application in the determination of DNA
1Department of Chemistry, Nankai University, Tianjin 300071, People's Republic of China.
Abstract:
The fluorescence energy transfer (FET) between Acridine Orange and Safranine T, two intercalators of DNA, was studied in this paper. The FET efficiency between Acridine Orange and Safranine T is higher and the critical distance, R(0), is longer in the intercalated state than in the free one. A new method for the determination of calf thymus DNA (ctDNA) was presented. The linear range of the calibration curve is (0 approximately 1.1)x10(-5) mol l(-1) in bases for ctDNA, and the limit of detection is 2.6x10(-7) mol l(-1).
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