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Updated: Jun 28, 2026

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
Published on: April 13, 2016
Chromium speciation using an automated liquid handling system with inductively coupled plasma - mass spectrometric
M K Donais1, R Henry, T Rettberg
1VG Elemental, 27 Forge Parkway, Franklin, MA 02038, USA.
Abstract:
The speciation of inorganic chromium in environmental samples is required for accurate assessment of pollution levels. Of the two chromium oxidation states, Cr (VI) is a known carcinogen, while Cr (III) is an essential element. Total chromium measurement cannot be used to determine actual environmental impact due to the considerable difference in toxicity of the two elemental forms. An automated liquid handling system, the PrepLabtrade mark, can be used with an inductively coupled plasma-mass spectrometer (ICP-MS) to quantify Cr (III) and Cr (VI) in liquid samples. An autosampler is used to introduce discrete sample volumes into a solid-phase chelation resin column. The Cr (III) and Cr (VI) species are separated and are introduced on-line into the VG PlasmaQuad 3 ICP-MS for detection. The chromatographic data are collected in time resolved analysis mode with the capability of simultaneous multiple-isotopic detection.
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