Related Experiment Video
Updated: Jun 28, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
Evaluation of phenolic assays for the detection of nitrite
J Davis1, K J McKeegan, M F Cardosi
1Department of Chemistry and Chemical Engineering, University of Paisley, Paisley, PA1 2BE, UK.
Abstract:
A series of generic nitrite assay systems based on the single step nitrosation of phenol derivatives are presented. The chemical reactivity offered by the C-nitroso compounds provides an opportunity to pursue a number of analytical strategies of which three spectroscopic (UV/Vis) and two electrochemical options (linear sweep voltammetry/differential pulse voltammetry) were evaluated. The capacity for multiple detection options from a single analyte species without significant sample manipulation is a major advantage with each assay system providing sub ppm detection limits with linear ranges up to milli-molar concentrations of nitrite. The influence of common interferents such as nitrate, ascorbate and paracetamol was investigated. The applicability of the assay procedures to the analysis of authentic biological samples (saliva and urine samples) was assessed with the analytical accuracy independently corroborated with a standard Griess protocol. In addition, a brief comparison with alternative nitrite detection strategies is also presented.
More Related Videos
08:23Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
08:25Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Related Concept Videos
Precipitation Titration: Endpoint Detection Methods
In the Volhard method, a standard excess of AgNO3 is first added to the...
2° Amines to N-Nitrosamines: Reaction with NaNO2