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Updated: Jun 28, 2026

Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
Transversely illuminated liquid core waveguide based fluorescence detection Fluorometric flow injection determination
1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061, USA.
Abstract:
The analytical performance of a new type of fluorescence detector, based on a transversely illuminated liquid core waveguide (LCW), has been investigated using the determination of NH(3)/NH(4)(+) as the 1-sulfonatoisoindole. With a very inexpensive combination of a miniature Hg blacklight as an excitation source, a colored plastic sheet as the emission filter, and an integrated blue sensitized photodiode-operational amplifier as the detector (totaling <$100 in hardware cost), we were able to achieve a limit of detection (LOD) of 35 nM (1.6 pmol) NH(3) with a linear dynamic range up to 60 muM NH(3). Details of detector construction and performance are given.
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