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Updated: Jun 28, 2026

Cortisol Measurement in Koala (Phascolarctos cinereus) Fur
Published on: August 23, 2019
Chromatographic characterization of a molecular imprinted polymer binding cortisol
C Baggiani1, G Giraudi, F Trotta
1Dipartimento di Chimica Analitica, Università degli Studi di Torino, Via Giuria 5, 10125 Turin, Italy.
Abstract:
A cortisol-binding polymer was prepared by utilising a non-covalent molecular imprinting polymerisation technique. The obtained polymer was packed in a high-performance liquid chromatography (HPLC) column; the selectivity was studied by liquid chromatography, eluting cortisol, cortisone, corticosterone, progesterone, 11-ketoprogesterone, 11alpha-hydroxyprogesterone, 17alpha-hydroxyprogesterone, cortisol 21-hemisuccinate, and cortisol 21-acetate with chloroform, containing 0.5% (v/v) acetic acid, as mobile phase. The mechanism of molecular imprinting was confirmed and a good selectivity for cortisol, with limited recognition for cortisone and 11alpha-hydroxyprogesterone, was found.
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