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Updated: Jun 28, 2026
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A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
Chemiluminescence as diagnostic tool. A review
C Dodeigne1, L Thunus, R Lejeune
1Service de Chimie Analytique, Institut de Pharmacie, Université de Liège, Avenue de l'Hôpital, 1, CHU, Tour 4, Niveau + 2, Sart Tilman, B-4000 Liege, Belgium.
Talanta
|October 31, 2008
Summary
Chemiluminescence, a light-emitting chemical reaction, offers powerful diagnostic tools. This review covers labels and applications in clinical chemistry, enhancing assays and biosensors for greater sensitivity.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Medical Diagnostics
Background:
- Chemiluminescence involves light emission from chemical reactions.
- Understanding luminescence and energy transfer is key to its application.
- Various chemiluminogenic labels exist, each with unique properties.
Purpose of the Study:
- To review the principles of chemiluminescence.
- To discuss its applications as a diagnostic tool in clinical chemistry.
- To highlight molecules suitable for modern diagnostic requirements.
Main Methods:
- Theoretical review of luminescence and energy transfer.
- Description of different chemiluminogenic label classes (luminol, acridinium, coelenterazine, dioxetanes, etc.).
- Discussion of applications in immunoassays, receptor assays, DNA probes, biosensors, and oxygen metabolism.
Main Results:
- Detailed overview of various chemiluminogenic labels and their suitability for clinical chemistry.
- Exploration of chemiluminescence and enhanced chemiluminescence applications in diverse diagnostic assays.
- Emphasis on the role of enzymes in achieving high selectivity and sensitivity.
Conclusions:
- Chemiluminescence is a versatile technology for clinical diagnostics.
- Careful selection of labels and understanding enzyme roles are crucial for assay optimization.
- Enhanced chemiluminescence offers significant advantages in sensitivity and selectivity for various biomedical applications.
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