Spectrofluorimetric determination of levofloxacin in tablets, human urine and serum

A spectrofluorimetric method to determine levofloxacin is proposed and applied to determine the substance in tablets and spiked human urine and serum. The fluorimetric method allow the determination of 20-3000 ng ml(-1) of levofloxacin in aqueous solution containing acetic acid-sodium acetate buffer (pH 4) with lambda(exc)=292 and lambda(em)=494 nm, respectively. Micelle enhanced fluorescence improves the sensibility and allows levofloxacin direct measurement in spiked Human serum (5 mug ml(-1)) and urine (420 mug ml(-1)), in 8 mM sodium dodecyl sulphate solutions at pH 5.