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Updated: Jun 28, 2026

Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions
Published on: November 12, 2017
FTIR spectroscopy studies of nucleic acid damage
G I Dovbeshko1, N Y Gridina, E B Kruglova
1Institute of Physics, National Academy of Sciences of Ukraine, 46 Prospekt Nauki, Kiev 03039, Ukraine.
Abstract:
Fourier transform infrared (FTIR) spectra of RNA isolated from tumour brain (glioma) and DNA isolated from low-dose gamma-irradiated epididymis cells of rats from the Chernobyl accident zone have been investigated in the 700-4000 cm(-1) region. The total absorbed radiation doses were equal to or less than 57 cGy. Observed changes in the FTIR spectra represent the damage in the primary, secondary and tertiary structure of nucleic acid, which seem to be connected with modification of bases and sugars, and redistribution of the H-bond network. FTIR data for tumour and gamma-irradiated nucleic acid show some similar features. A great amount of statistical data and good mathematical approaches are needed for the use of these data as diagnostic criteria.
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