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High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

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The sequential injection system with adsorptive stripping voltammetric detection.

W W Kubiak¹, R M Latonen, A Ivaska

¹Faculty of Materials Science and Ceramic, University of Mining and Metallurgy, al. Mickiewicza 30, PL 30-059 Krakow, Poland.

|October 31, 2008

Summary

Developed sequential injection systems enable sensitive adsorptive stripping voltammetry for riboflavin and metal ion determination. These methods offer high reproducibility and low detection limits for applications in food analysis and environmental monitoring.

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Area of Science:

Analytical Chemistry
Electrochemistry

Background:

Adsorptive stripping voltammetry (AdSV) is a sensitive electroanalytical technique.
Sequential injection analysis (SIA) offers automation and reduced sample consumption.

Purpose of the Study:

To develop and validate two sequential injection systems for adsorptive stripping voltammetric measurements.
To determine riboflavin and various metal ions with high sensitivity and reproducibility.

Main Methods:

Development of two sequential injection systems for AdSV.
System 1: Direct aspiration for mercury-adsorbing substances (e.g., riboflavin).
System 2: Sample and ligand aspiration for complexing metal ions prior to deposition.

Main Results:

System 1 achieved 3% reproducibility and a 0.07 µM detection limit for riboflavin.
System 2 achieved <0.1 ppm detection limits for Cd, Pb, and Cu using oxine.
Lower detection limits (<0.01 ppm) were achieved for Ni and Co using dimethylglyoxime and nioxime.
Simultaneous determination of multiple metal ions (e.g., Cu, Pb, Cd, Co) was demonstrated.

Conclusions:

The developed SIA-AdSV systems provide efficient and sensitive analytical methods.
These systems are applicable for determining riboflavin in vitamin pills and studying its photodegradation.
The methods are suitable for trace metal ion analysis in environmental samples and complex matrices.