Study on the aggregation of teicoplanin.
E Tesarová1, Z Tuzar, K Nesmerák
1Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Albertov 2030, 12840, Prague 2, Czech Republic.
Talanta
|October 31, 2008
Summary
Teicoplanin aggregation and micelle formation were investigated. Acetonitrile addition prevented micelle formation, indicating different solvation and association mechanisms.
Area of Science:
- Pharmaceutical Sciences
- Physical Chemistry
Background:
- Teicoplanin is a complex glycopeptide antibiotic.
- Understanding teicoplanin's aggregation behavior is crucial for its formulation and efficacy.
- Solvent effects on drug aggregation are significant.
Purpose of the Study:
- To investigate the aggregation of teicoplanin in different buffer solutions.
- To determine the critical micelle concentration (CMC) of teicoplanin.
- To elucidate the influence of acetonitrile on teicoplanin's self-assembly.
Main Methods:
- Conductometry
- Capillary electrophoresis
- Cyclic voltammetry
- Static light scattering
- Dynamic light scattering
Main Results:
- Teicoplanin exhibited critical micelle concentration (CMC) in phosphate buffers.
- No CMC was detected in solutions containing 10% acetonitrile.
- Particle size and distribution were analyzed using dynamic light scattering.
Conclusions:
- Acetonitrile significantly alters teicoplanin's aggregation behavior.
- Different solvation mechanisms are proposed for solutions with and without acetonitrile.
- Two distinct association schemes were identified based on solvent composition.
