Prussian Blue bulk modified screen-printed electrodes for H(2)O(2) detection and for biosensors.

Area of Science:

• Electrochemistry
• Biosensors
• Materials Science

Background:

• Hydrogen peroxide (H2O2) is a key analyte in various biological and chemical processes.
• Amperometric sensors offer sensitive detection but often face challenges with selectivity and stability.
• Prussian Blue (PB) is a promising material for electrochemical sensing applications due to its redox properties.

Purpose of the Study:

• To develop and optimize a Prussian Blue (PB) bulk modified carbon screen-printed electrode for H2O2 amperometric detection.
• To evaluate the performance of the developed sensor, including its limit of detection, linear range, and sensitivity.
• To adapt the PB-modified electrode as a transducer for a glucose biosensor and assess its performance.

Main Methods:

• Chemical synthesis of Prussian Blue (PB) powder via FeCl3 and K4[Fe(CN)6] reaction.
• Preparation of PB microparticles (<38 µm) and their incorporation into carbon ink for screen-printed electrodes.
• Amperometric detection of H2O2 and glucose using the developed modified electrodes.
• Optimization of the sensor for the lowest limit of detection.

Main Results:

• The H2O2 sensor achieved a limit of detection (LOD) of 0.4 µM and a linear range up to 100 µM.
• Sensitivity for H2O2 detection was 137 µA mM⁻¹ cm⁻², comparable to electrodeposited PB films.
• The glucose biosensor exhibited an LOD of 0.22 mM, a linear range up to 3 mM, and a sensitivity of 3.21±0.16 µA mM⁻¹ cm⁻².
• Both sensors demonstrated high insensitivity to oxygen, ascorbate, urate, and paracetamol.

Conclusions:

• A cost-effective and efficient Prussian Blue (PB) bulk modified carbon screen-printed electrode was successfully developed for H2O2 detection.
• The developed transducer is suitable for constructing a sensitive and selective glucose biosensor.
• The sensor's robustness against common interferents highlights its potential for practical applications.