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Published on: December 20, 2016
Simultaneous determination of nitrite and nitrate by normal phase ion-pair liquid chromatography
1Central Analytical Facility, PINSTECH, P.O. Nilore, Islamabad, Pakistan.
Abstract:
Normal phase ion-pair high performance liquid chromatography has been used for simultaneous separation of nitrite and nitrate using tetraethylammonium (TEA)(+) as ion-pairing reagent. The concentration effect of (TEA)(+), buffer salt and pH of the eluent on separation is investigated. The UV detector response at various wavelengths has been optimized. The performance of the proposed method is compared with ion chromatography for quantification of the anions in potable water, wastewater and in food samples, such as spinach and lettuce.
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