Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An evaluation of continuous subcutaneous infusions across seven NHS acute hospitals: is there potential for 48-hour infusions?

BMC palliative care·2020

Same author

The increased risk of microvascular complications in South Asians with type 1 diabetes is influenced by migration.

Diabetic medicine : a journal of the British Diabetic Association·2019

Same author

3D Bioprinting:principles, fantasies and prospects.

Journal of stomatology, oral and maxillofacial surgery·2019

Same author

Central nervous system involvement in multiple symmetric lipomatosis.

Journal of the neurological sciences·2016

Same author

Surface Functionalization for Immobilization of Probes on Microarrays.

Methods in molecular biology (Clifton, N.J.)·2015

Same author

Patterns of IgE sensitization in house dust mite-allergic patients: implications for allergen immunotherapy.

Allergy·2015

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Impedance based DNA chip for direct T(m) measurement.

C A Marquette¹, I Lawrence, C Polychronakos

¹Department of Chemistry and Biochemistry, Concordia University, 1455 de Maisonneuve West, Montreal, Quebec, Canada H3G 1M8.

|October 31, 2008

Summary

This study developed a silicon chip sensor for detecting DNA hybridization using impedance measurements. The optimized sensor accurately determined the DNA melting temperature (Tm), showing promise for molecular diagnostics.

More Related Videos

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

Published on: May 18, 2013

Related Experiment Videos

Last Updated: Jun 28, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

Published on: May 18, 2013

Area of Science:

Materials Science
Biotechnology
Analytical Chemistry

Background:

Impedance measurement offers a label-free detection method for biomolecular interactions.
Developing sensitive and specific DNA hybridization sensors is crucial for diagnostics.

Purpose of the Study:

To develop and optimize a silicon-based impedance sensor for detecting DNA hybridization.
To determine the melting temperature (Tm) of immobilized oligonucleotides using impedance spectroscopy.

Main Methods:

Immobilization of Oligo d(T)(20) on Si/SiO(2) chips via aminopropyl silane and glutaraldehyde activation.
Detection of DNA hybridization by measuring impedance changes at -300 mV DC potential.
Determination of Tm using impedance measurements in a temperature-controlled flow system.

Main Results:

Optimized immobilization yielded a 50 Omega impedance change upon hybridization.
The sensor detected a reproducible 65 Omega impedance change at 32 degrees C for Tm determination.
The experimental Tm value closely matched the theoretically calculated value.

Conclusions:

The developed Si/SiO(2) chip sensor effectively detects DNA hybridization via impedance changes.
The sensor accurately determines the melting temperature of immobilized DNA, demonstrating specificity and reproducibility.
This impedance-based approach shows potential for label-free, real-time DNA analysis.