Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MOrbVis: Browser-Based Molecular Orbital Visualization with WebGPU-Accelerated On-the-Fly Evaluation.

ACS omega·2026
Same author

The Quantum Optimization Benchmarking Library.

Nature computational science·2026
Same author

Recurrent Upper Extremity Deep Vein Thrombosis Due to Paget-Schroetter Syndrome.

Circulation journal : official journal of the Japanese Circulation Society·2026
Same author

Nucleic acid detection based on single-cluster analysis of cross-linking aggregates of DNA-modified gold nanoparticles using a dark-field microscope.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026
Same author

Assessment of reinforcing sutures of double-stapling anastomosis with manual and powered circular staplers in Porcine models.

Scientific reports·2026
Same author

Enhancing the Milan System for Reporting Salivary Gland Cytology Through Ancillary Testing Using Liquid-Based Cytology.

Cytopathology : official journal of the British Society for Clinical Cytology·2026

Related Experiment Video

Updated: Jun 28, 2026

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
07:07

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Published on: March 12, 2015

DNA sensors using a ferrocene-oligonucleotide conjugate.

Masamichi Nakayama1, Toshihiro Ihara, Koji Nakano

  • 1Department of Materials Physics and Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 812-8581, Japan.

Talanta
|October 31, 2008
PubMed
Summary

A novel electrochemical gene sensor uses a ferrocene-modified DNA probe for sensitive detection. This method can distinguish single-base DNA mutations without radioisotopes, offering a convenient alternative for genetic analysis.

More Related Videos

A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Detection of Bacteria Using Fluorogenic DNAzymes
13:20

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Related Experiment Videos

Last Updated: Jun 28, 2026

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
07:07

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Published on: March 12, 2015

A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Detection of Bacteria Using Fluorogenic DNAzymes
13:20

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Area of Science:

  • Biotechnology
  • Electrochemistry
  • Molecular Biology

Background:

  • Developing sensitive and isotope-free methods for DNA detection is crucial for genetic analysis.
  • Existing gene sensors often rely on radioisotopes or complex procedures.

Purpose of the Study:

  • To develop a novel, sensitive, and convenient electrochemical gene sensor.
  • To demonstrate the sensor's ability to detect single-base DNA mutations.

Main Methods:

  • Immobilization of a 16-mer oligonucleotide probe onto a gold electrode via phosphorothioate chemisorption.
  • Utilizing a ferrocene-modified 12-mer oligonucleotide conjugate as a redox probe.
  • Formation of a sandwich-type ternary complex between the electrode-bound probe, target DNA, and the ferrocene conjugate.

Main Results:

  • The electrochemical gene sensor demonstrated high sensitivity and convenience.
  • The system successfully distinguished between a fully complementary target DNA and a mutant with a single base substitution.
  • Electric currents generated by ferrocene oxidation provided a measurable signal.

Conclusions:

  • The developed electrochemical gene sensor is a promising tool for sensitive and specific DNA mutation detection.
  • This non-radioisotopic method offers a practical alternative for genetic diagnostics and research.
  • The sandwich assay format combined with redox probes provides a robust platform for gene sensing.