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Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

Gas Chromatography: Sample Injection Systems

Gas Chromatography: Sample Injection Systems

In gas chromatography, the sample is introduced as a vapor plug into the carrier gas stream for high efficiency and resolution. A microsyringe injects the sample solution into a heated sample port, vaporizing it and mixing it with the carrier gas. This process is important to ensure the sample is properly prepared for analysis. Thermally sensitive samples can be injected directly into the column and volatilized by slowly increasing the column temperature.
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Gas Chromatography: Types of Detectors-I

Gas Chromatography: Types of Detectors-I

There are different types of detectors used in gas chromatography, each with its own specific properties that make it suitable for detecting certain types of analytes. The most commonly used detectors in GC are thermal conductivity detector (TCD), flame ionization detector (FID), and electron capture detector (ECD).
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...

Gas Chromatography: Types of Detectors-II

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...

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Related Experiment Video

Updated: Jun 28, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

Flow injection microbial trichloroethylene sensor.

Tae-Sung Han¹, Satoshi Sasaki, Kazuyoshi Yano

¹Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.

|October 31, 2008

Summary

A new microbial biosensor detects trichloroethylene (TCE) in water. This flow-through sensor uses Pseudomonas aeruginosa JI104 and electrical detection for accurate, real-time monitoring of TCE contamination.

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Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

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Last Updated: Jun 28, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

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Published on: October 15, 2015

Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity

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Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Published on: January 3, 2012

Area of Science:

Environmental Science
Analytical Chemistry
Biotechnology

Background:

Trichloroethylene (TCE) is a common environmental pollutant.
Accurate and rapid detection methods for TCE are crucial for water quality monitoring.
Existing methods may be time-consuming or lack field applicability.

Purpose of the Study:

To develop a novel flow-type microbial biosensor for direct measurement of TCE.
To utilize the bacterium Pseudomonas aeruginosa JI104 for TCE degradation and chloride ion release detection.
To optimize sensor performance for reliable TCE quantification.

Main Methods:

Development of a flow cell biosensor using glass to prevent TCE adsorption and vaporization.
Immobilization of TCE-degrading bacterium Pseudomonas aeruginosa JI104.
Electrical detection of chloride ions released during microbial TCE degradation.
Evaluation of sensor performance based on carrier solution pH, microbe amount, flow rate, and injection volume.

Main Results:

The microbial biosensor demonstrated linear response to TCE concentrations from 0.03 to 2 mgl(-1).
Optimized parameters ensured reliable sensor performance.
The system showed good response in real groundwater samples, confirming its applicability.

Conclusions:

The developed flow-type microbial biosensor offers a sensitive and direct method for TCE detection.
The sensor is suitable for on-site, real-time monitoring of TCE in water.
This technology has potential applications in assessing drinking water safety and monitoring contaminated sites.