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Published on: December 16, 2019
Chemiluminescence flow-through sensor for ofloxacin using solid-phase PbO(2) as an oxidant
Baoxin Li1, Zhujun Zhang, Lixia Zhao
1Department of Chemistry, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, People's Republic of China.
Talanta
|October 31, 2008
Summary
A new chemiluminescence sensor detects ofloxacin using sulfite oxidation. This reusable sensor offers a sensitive method for analyzing ofloxacin in pharmaceutical preparations.
Area of Science:
- Analytical Chemistry
- Electrochemistry
- Sensor Technology
Background:
- Ofloxacin is a widely used antibiotic.
- Accurate quantification of ofloxacin in pharmaceutical preparations is crucial.
- Existing analytical methods may have limitations in sensitivity or reusability.
Purpose of the Study:
- To develop a novel chemiluminescence (CL) flow-through sensor for the sensitive determination of ofloxacin.
- To investigate the use of immobilized lead dioxide (PbO2) as a reusable oxidant in the CL system.
- To validate the sensor's performance for analyzing ofloxacin in pharmaceutical samples.
Main Methods:
- A chemiluminescence sensor was designed based on the sensitizing effect of ofloxacin on the CL oxidation of sulfite by lead dioxide (PbO2) in sulfuric acid (H2SO4) media.
- Solid-phase PbO2 was immobilized within the CL flow cell, serving as the immobilized CL oxidant.
- The performance of the sensor was evaluated using standard solutions of ofloxacin, and its applicability was tested on pharmaceutical preparations.
Main Results:
- The immobilized PbO2 column demonstrated excellent reusability, functioning for approximately 400 cycles over 50 hours.
- The calibration graph for ofloxacin was linear within the concentration range of 0.2-10 µg/mL.
- A low detection limit of 7.8x10⁻⁸ g/mL (Signal-to-Noise ratio = 3) was achieved.
- The method showed successful application in determining ofloxacin content in pharmaceutical preparations.
Conclusions:
- A novel, reusable chemiluminescence flow-through sensor for ofloxacin determination has been successfully developed.
- The sensor utilizes the sensitizing effect of ofloxacin on sulfite oxidation by immobilized PbO2, offering high sensitivity and a low detection limit.
- This method provides a robust and efficient approach for the quality control of ofloxacin in pharmaceutical formulations.
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