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Related Experiment Video

Updated: Jun 28, 2026

A Method to Preserve Wetland Roots and Rhizospheres for Elemental Imaging

A Method to Preserve Wetland Roots and Rhizospheres for Elemental Imaging

Published on: February 15, 2021

Arsenic speciation in contaminated soils.

S Garcia-Manyes¹, G Jiménez, A Padró

¹Departament de Quimica Analitica, Universitat de Barcelona, Avda. Diagonal, 647, E-08028 Barcelona, Spain; Departament de Quimica Fisica, Universitat de Barcelona, Avda. Diagonal, 647, E-08028 Barcelona, Spain.

|October 31, 2008

Summary

A new method accurately determines arsenic species in soils using liquid chromatography-hydride generation-inductively coupled plasma mass spectrometry. This analysis reveals arsenate as the primary species in contaminated Spanish soils.

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Integrated Field Lysimetry and Porewater Sampling for Evaluation of Chemical Mobility in Soils and Established Vegetation

Integrated Field Lysimetry and Porewater Sampling for Evaluation of Chemical Mobility in Soils and Established Vegetation

Published on: July 4, 2014

Area of Science:

Environmental Chemistry
Analytical Chemistry
Soil Science

Background:

Arsenic contamination in agricultural soils poses environmental and health risks.
Accurate speciation of arsenic is crucial for understanding its mobility and bioavailability.
Existing methods may lack efficiency or comprehensive analysis for diverse arsenic forms.

Purpose of the Study:

To develop and validate a robust method for arsenic speciation in contaminated soils.
To investigate the stability of arsenic species during the extraction process.
To compare the developed speciation method with standard extraction techniques.

Main Methods:

Extraction using a mixture of phosphoric acid and ascorbic acid.
Speciation analysis via liquid chromatography (LC)-ultraviolet (UV) irradiation-hydride generation (HG)-inductively coupled plasma mass spectrometry (ICP/MS).
Validation using certified reference materials (CRMs) and comparison with aqua regia extraction (ISO Standard 11466).

Main Results:

The developed LC-UV-HG-ICP/MS method successfully speciated arsenic in Spanish agricultural soils.
Arsenate was identified as the predominant arsenic species in analyzed soils.
Arsenite and methylated arsenic species were detected in some samples, indicating complex contamination.
The LC-UV-HG coupled with atomic fluorescence spectrometry (AFS) also proved effective for arsenic speciation.

Conclusions:

The developed extraction and LC-UV-HG-ICP/MS method provides accurate arsenic speciation in contaminated soils.
The method is reliable for identifying major and minor arsenic species, including arsenate, arsenite, and methylated forms.
Alternative detection with AFS offers a viable and user-friendly option for arsenic speciation analysis.