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Resonance light scattering technique for the determination of proteins with resorcinol yellow and OP
Yan-Jing Chen1, Jing-He Yang, Xia Wu
1Laboratory of Colloid and Interface Chemistry for Educational Ministry, Department of Chemistry, Shandong University, Jinan 250100, People's Republic of China; Department of Chemistry, Jinan University, Jinan 250002, People's Republic of China.
Abstract:
A new resonance light scattering (RLS) assay of proteins is presented. In the citric acid-NaOH (pH 2.35) buffer, the RLS of Resorcinol yellow (RY)-protein system can be greatly enhanced by addition of nonionic surfactant OP, owing to the interaction between OP and RY-protein. The enhanced RLS is in proportion to the concentration of proteins in the range 0.02-4.0 mug ml(-1) for both bovine serum albumin (BSA) and bovine hemoglobin (HEM), the detection limits were 10.4 ng ml(-1) (S/N=3) for BSA and 11.4 ng ml(-1) (S/N=3) for HEM. Samples were determined satisfactorily.
