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Updated: Jun 28, 2026

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
Published on: May 19, 2023
SOLVERSTAT: a new utility for multipurpose analysis. An application to the investigation of dioxygenated Co(II)
Clara Comuzzi1, Pierluigi Polese, Andrea Melchior
1Dipartimento di Scienze e Tecnologie Chimiche, Università di Udine, Via Cotonificio 108, I-33100 Udine, Italy.
Abstract:
A new utility for multipurpose analysis, SOLVERSTAT, taking advantage of the versatility of spreadsheets is here described. By means of this tool advanced statistical tests have introduced in Microsoft Excel Solver thus allowing regression diagnostic and discrimination between different models. The utility is here applied to the determination, by UV-Vis spectroscopy, of the stability constant for the uptake of molecular dioxygen by the 1:2 complex of Co(II) with N,N'-dimethylethylenediamine (dmen) in the aprotic solvent dimethylsulfoxide (dmso) at 298 K and in a medium adjusted to 0.1 mol dm(-3) with Et(4)NClO(4). The reliability of the model and parameters obtained are discussed and the results compared with those obtained by Dynafit, a different software package, and by independent voltammetric measurements. The validity of SOLVERSTAT has been also examined applying it to the discrimination between different models already discussed in the literature.
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