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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Analysis of promazines in bovine livers by high performance liquid chromatography with ultraviolet and fluorimetric
M C Quintana1, M H Blanco, J Lacal
1Department of Analytical Chemistry and Instrumental Analysis, Faculty of Sciences, Universidad Autónoma de Madrid, E-28049 Madrid, Spain.
Abstract:
Fluorimetric and UV methods of detection for liquid chromatographic determination of phenothiazines (chlorpromazine, acetopromazine and propionylpromazine) were developed. The effects of several experimental parameters on the separation and the sensitivity of the methods were evaluated. The detection limits ranged from 31 to 350 ngml(-1). Optimized methods were successfully applied to determination of the promazines in bovine liver samples.

