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Determination of the binding constant for the inclusion complex between procaine hydrochloride and beta-cyclodextrin
Nianbing Li1, Jianping Duan, Hongqing Chen
1Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, PR China.
Abstract:
The apparent electrophoretic mobilities of procaine hydrochloride (mu(i)) in a series of concentration of beta-cyclodextrin were measured directly by capillary electrophoresis technology. A new mathematical treatment method is proposed, which based on the fact that the molar ratio of the inclusion complex was 1:1 established by spectrophotometry. Using the proposed method, the binding constant of the inclusion complex of procaine hydrochloride with beta-cyclodextrin can be obtained easily. The determination result was in correspondence with those of the spectrophotometric and fluorescence methods.
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