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Updated: Jun 28, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
Published on: May 5, 2016
Light-emitting-diode-induced fluorescence detector for capillary electrophoresis using optical fiber with spherical
1Department of Analytical Chemistry and Micro-Instrumentation, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, P.O. Box 100, Dalian 116012, People's Republic of China.
Abstract:
A simple fluorescence detector for capillary electrophoresis (CE) using a blue light-emitting-diode (LED) as excitation source is constructed and evaluated. An optical fiber was used to collect the fluorescence, and a flat end of the fiber was modified to spherical end, resulting in 50% increase of efficiency over the flat end. A simple device for optical alignment of the fibers and capillary column was designed. The concentration and mass detection limits for fluorescein were 1.8x10(-7) mol l(-1) and 4.3 femol, respectively.

