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Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
Published on: July 4, 2011
Flow-through bulk optode for the fluorimetric determination of perchlorate
J A Ortuño1, M I Albero, M S García
1Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia, Spain.
Abstract:
A flow-through fluorescence bulk optode for the flow-injection determination of perchlorate is described. As the active constituents the optode incorporates the lipophilized pH indicator fluorescein octadecyl ester and methyl tridodecyl ammonium chloride, dissolved in a plasticized poly (vinyl) chloride membrane entrapped in a cellulose support. The optode is applied in conjunction with the flow injection technique for perchlorate determination at pH 4.5 (acetic-acetate). The sensor is readily regenerated with the pH 10.4 (TRIS) carrier solution. The analytical characteristics of this optode with respect to perchlorate response time, dynamic measurement range, reproducibility and selectivity are discussed. The proposed FI method is applied to the determination of perchlorate in waters from different sources.
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