Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

A surface plasmon resonance immunosensor for detecting a dioxin precursor using a gold binding polypeptide.

Nobuaki Soh¹, Tomoyuki Tokuda, Tomomi Watanabe

¹Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

|October 31, 2008

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mesopore-Depth Optimization in Mesoporous Gold Films for SF<sub>6</sub> Electroreduction.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Characterisation of a cold-adapted, thermostable glucokinase from psychrophilic Pseudoalteromonas sp. AS-131 reveals how the enzyme achieves high thermal stability without loss of cold adaptation.

The FEBS journal·2025

Same author

[Hoehn & Yahr scale and MDS-UPDRS Part 3 progression in Parkinson's disease patients: retrospective natural history study using a disease registry].

Rinsho shinkeigaku = Clinical neurology·2025

Same author

The efficacy of orbital atherectomy is associated with prior rotational atherectomy and a greater preprocedural lumen area.

Coronary artery disease·2025

Same author

Sequential Injection Analysis of Cholesterol Using an Oxidation-Reduction Electrode Detector.

Sensors (Basel, Switzerland)·2025

Same author

Utility and feasibility of intra-pocket mapping technique for optimal subcutaneous implantable cardioverter defibrillator implantation positioning for R-wave detection: a case series.

European heart journal. Case reports·2025

A new surface plasmon resonance (SPR) biosensor effectively monitors 2,4-dichlorophenol, a dioxin precursor. The indirect competitive immunoassay method achieved a sensitive detection limit of 20 ppb, significantly improving upon direct methods.

Area of Science:

Biosensing
Analytical Chemistry
Environmental Monitoring

Background:

2,4-dichlorophenol is a significant dioxin precursor requiring sensitive detection methods.
Surface Plasmon Resonance (SPR) offers label-free detection capabilities for biomolecular interactions.
Traditional direct immunoassay methods may lack the required sensitivity for trace contaminant monitoring.

Purpose of the Study:

To develop and optimize an SPR-based biosensor for the sensitive detection of 2,4-dichlorophenol.
To compare the performance of direct and indirect competitive immunoassay formats for 2,4-dichlorophenol monitoring.
To evaluate the role of gold binding polypeptide (GBP) in enhancing antibody immobilization and sensor sensitivity.

Main Methods:

Fabrication of an SPR sensor chip with immobilized anti-(2,4-dichlorophenol) antibody using gold binding polypeptide (GBP) and protein G.

More Related Videos

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Published on: January 3, 2012

Related Experiment Videos

Last Updated: Jun 28, 2026

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Published on: January 3, 2012

Implementation of an indirect competitive immunoassay format using 2,4-dichlorophenol conjugated with bovine serum albumin (BSA).

Measurement of SPR response upon injection of varying concentrations of 2,4-dichlorophenol in the flow system.

Main Results:

Direct immunoassay showed minimal SPR response (<0.001 degrees shift) for 25 ppm 2,4-dichlorophenol.
The indirect competitive immunoassay demonstrated high sensitivity, achieving an estimated detection limit of 20 ppb.
Utilizing GBP for antibody immobilization significantly enhanced sensor sensitivity compared to using BSA.

Conclusions:

The developed SPR biosensor with an indirect competitive immunoassay is highly sensitive for 2,4-dichlorophenol detection.
GBP-mediated antibody immobilization is crucial for achieving enhanced sensitivity in SPR biosensing applications.
This method provides a promising tool for monitoring dioxin precursors in environmental samples.