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Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Published on: January 3, 2012
An optical surface plasmon resonance biosensor for determination of tetanus toxin
Xia Liu1, Daqian Song, Qinglin Zhang
1College of Chemistry, Jilin University, Changchun 130023, PR China.
Abstract:
Surface plasmon resonance (SPR) has been successfully applied for the simple, rapid, and label-free assay of various biomolecules. This assay evaluates a novel wavelength modulation SPR biosensor for the detection of tetanus toxin. The wavelength modulation SPR biosensor is designed based on fixing the incident angle of light and measuring the reflected intensities in the resonance wavelength range spanning 400-800nm simultaneously. Tetanus toxin (TeNT), one of the most potent toxins known, is synthesized as a 150kDa single polypeptide chain. The SPR biosensor has been shown to be capable of directly detecting concentration of tetanus toxin as low as 0.028Lfml(-1). Under selected experimental conditions, the SPR biosensor has a good reproducibility, sensitivity and reversibility. The results illustrate how wavelength modulation SPR biosensor can be used to detect biomolecular interactions.
