Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

Controlled-Potential Coulometry: Electrolytic Methods

Controlled-Potential Coulometry: Electrolytic Methods

Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential ensures...

Controlled-Current Coulometry: Overview

Controlled-Current Coulometry: Overview

Controlled current coulometry, also known as amperostatic coulometry, is a technique used in electrochemical analysis to measure the quantity of a substance through the controlled passage of current. It involves the application of a constant current to an electrochemical cell containing the analyte of interest. As the current flows through the cell, the analyte undergoes a redox reaction at the electrode surface, resulting in a charge transfer. By monitoring the time required for a certain...

Thermal and Photochemical Electrocyclic Reactions: Overview

Thermal and Photochemical Electrocyclic Reactions: Overview

Electrocyclic reactions are reversible reactions. They involve an intramolecular cyclization or ring-opening of a conjugated polyene. Shown below are two examples of electrocyclic reactions. In the first reaction, the formation of the cyclic product is favored. In contrast, in the second reaction, ring-opening is favored due to the high ring strain associated with cyclobutene formation.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Prenatal Diagnosis of Caroli's Disease by Ultrasound and MRI Imaging.

Prenatal diagnosis·2025

Same author

[Randomized,double-blind,parallel controlled,multicenter clinical trial of effectiveness and safety of Shexiang Zhuifeng Zhitong Ointment in alleviating pain in knee osteoarthritis(syndrome of cold-dampness obstruction)].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·2024

Same author

Phosphatidic acid interacts with an HD-ZIP transcription factor GhHOX4 to influence its function in fiber elongation of cotton (Gossypium hirsutum).

The Plant journal : for cell and molecular biology·2024

Same author

The high-affinity transporter BnPHT1;4 is involved in phosphorus acquisition and mobilization for facilitating seed germination and early seedling growth of Brassica napus.

BMC plant biology·2019

Same author

Characterization of bHLH/HLH genes that are involved in brassinosteroid (BR) signaling in fiber development of cotton (Gossypium hirsutum).

BMC plant biology·2018

Same author

Identification of hsa-mir-34a, hsa-mir-124, and hsa-mir-204 as signatures for cataract.

Journal of cellular physiology·2018

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)

Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)

Published on: June 14, 2021

Electroanalytical method of TCPP and its supramolecular system with cyclodextrins.

Yun Liu¹, Jing-Hao Pan, Ying-Li Wei

¹Chemistry Department, School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.

|October 31, 2008

Summary

A new electroanalytical method for tetrakis (4-carboxylphenyl) porphyrin (TCPP) was developed using oscillopolarography. This method accurately quantifies TCPP and studies its inclusion complexes with various cyclodextrins (CDs), revealing strong interactions with modified CDs.

More Related Videos

OnePot PURE Cell-Free System

OnePot PURE Cell-Free System

Published on: June 23, 2021

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Published on: May 4, 2018

Related Experiment Videos

Last Updated: Jun 28, 2026

Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)

Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)

Published on: June 14, 2021

OnePot PURE Cell-Free System

OnePot PURE Cell-Free System

Published on: June 23, 2021

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Published on: May 4, 2018

Area of Science:

Analytical Chemistry
Electrochemistry
Supramolecular Chemistry

Background:

Tetrakis (4-carboxylphenyl) porphyrin (TCPP) is a molecule with potential applications.
Understanding its interactions with other molecules is crucial for further development.
Cyclodextrins (CDs) are known for their ability to form inclusion complexes.

Purpose of the Study:

To establish a sensitive electroanalytical method for TCPP detection.
To investigate the supramolecular interactions between TCPP and various cyclodextrins.
To determine the formation constants of these inclusion complexes.

Main Methods:

Single-sweep oscillopolarography was employed to develop the electroanalytical method for TCPP.
Polarography was used to study the interaction of TCPP with five different cyclodextrins (beta-CD, gamma-CD, hydroxylpropyl-beta-CD, sulfurbutylether-beta-CD, and trimethyl-beta-CD).
The formation constants of the TCPP-CD inclusion complexes were determined.

Main Results:

A sensitive second derivative reduction peak for TCPP was identified at -0.70V (vs. SCE) in a phosphate buffer (pH 7.0).
A linear relationship between peak height and TCPP concentration was observed from 1x10(-7) to 2x10(-5) mol/L, with good precision (R.S.D. 0.41%) and recovery (95.8-105.4%).
TCPP forms 1:1 inclusion complexes with all tested CDs, with hydroxylpropyl-beta-CD showing the strongest inclusion ability. Modified CDs demonstrated higher capacity than native beta-CD, and gamma-CD exhibited a greater formation constant than beta-CD due to cavity size compatibility.

Conclusions:

An effective electroanalytical method for TCPP determination has been successfully established.
TCPP forms stable inclusion complexes with various cyclodextrins, particularly modified ones.
The study provides valuable supramolecular data for the future applications of TCPP.