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Sequential injection spectrophotometric determination of phenylephrine hydrochloride in pharmaceutical preparations
Negussie W Beyene1, Jacobus F Van Staden
1Department of Chemistry, University of Pretoria, Pretoria 0002, South Africa.
Abstract:
A fully automated sequential injection spectrophotometric method for the determination of phenylephrine hydrochloride in pharmaceutical preparations is reported. The method is based on the condensation reaction of the analyte with 4-aminoantipyrine in the presence of potassium ferricyanide. The absorbance of the condensation product was monitored at 503nm. A linear relationship between the relative peak height and concentration was obtained in the range 0.5-17.5mgl(-1). The detection limit (as 3sigma value) was 0.09mgl(-1) and repeatability was 0.8 and 0.6% at 2.5 and 5mgl(-1), respectively. Results obtained by this method agreed very well with those obtained by the AOAC official method.
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