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Updated: Jun 28, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
Preparation and evaluation of non-bonded hyperbranched polymer-coated columns for capillary electrophoresis
Chong-Qi Shou1, Chang-Li Zhou, Chun-Bin Zhao
1Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China; Department of Applied Chemistry, Jinan University, Jinan 250022, PR China.
Abstract:
A series of hyperbranched poly(amine-ester)s based on 1,1,1-trimethylolpropane, methyl acrylate and diethanolamine were synthesized and coated on the inner surface of the fused-silica capillaries by physical adsorption. The most effective coating was the seventh generation hyperbranched poly(amine-ester) coating, which reduced the electroosmotic flow (EOF) greatly and suppressed protein adsorption effectively. The high separation efficiencies for basic proteins were obtained and the coating had a good stability.
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