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IR and UV–Vis Spectroscopy of Carboxylic Acids01:28

IR and UV–Vis Spectroscopy of Carboxylic Acids

In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency, 1710 cm−1. The C=O bond of the...

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Selective spectrofluorimetric method for paracetamol determination through coumarinic compound formation.

María de los A Oliva1, Roberto A Olsina, Adriana N Masi

  • 1Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Chacabuco y Pedernara, San Luis, Argentina. mdoliva@unsl.edu.ar

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Summary

A new spectrofluorimetrical method accurately determines paracetamol in tablets. This sensitive, simple, and cost-effective technique offers a viable alternative for pharmaceutical quality control.

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Area of Science:

  • Analytical Chemistry
  • Pharmaceutical Analysis

Background:

  • Paracetamol is a widely used analgesic and antipyretic.
  • Existing methods for paracetamol determination can be complex and costly.
  • There is a need for efficient and economical analytical techniques in pharmaceutical quality control.

Purpose of the Study:

  • To develop a selective spectrofluorimetrical method for paracetamol determination.
  • To establish a cost-effective and sensitive analytical approach for paracetamol in pharmaceutical formulations.
  • To optimize the method using chemometric techniques.

Main Methods:

  • Reaction of paracetamol with ethylacetoacetate (EAA) in the presence of sulfuric acid to form a fluorescent coumarinic compound.
  • Spectrofluorimetrical measurement of the product's fluorescence at 478 nm (excitation at 446 nm).
  • Optimization of reaction variables using chemometric analysis.

Main Results:

  • The method demonstrated high sensitivity with a linear concentration range of 0.1-0.4 µg/mL and a detection limit of 57 ng/mL.
  • The developed spectrofluorimetrical technique proved effective for analyzing paracetamol in pharmaceutical formulations.
  • The method is characterized by its simplicity, speed, and lower cost compared to official methods.

Conclusions:

  • The proposed spectrofluorimetrical method is a selective, sensitive, and economical approach for paracetamol determination.
  • This technique is suitable for routine pharmaceutical quality control and medicament analysis.
  • The method offers a profitable and efficient alternative to current official analytical procedures.