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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

Updated: Jun 28, 2026

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
10:01

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

A two-color-change, nanoparticle-based method for DNA detection.

Y Charles Cao1, Rongchao Jin, C Shad Thaxton

  • 1Department of Chemistry and Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.

Talanta
|October 31, 2008
PubMed
Summary
This summary is machine-generated.

We developed novel silver/gold core-shell nanoparticles for DNA detection. These probes offer distinct optical properties, enabling a two-color method for identifying single-nucleotide polymorphisms in DNA targets.

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Area of Science:

  • Nanotechnology
  • Biochemistry
  • Molecular Biology

Background:

  • Silver/gold core-shell nanoparticles combine silver's optical properties with gold's functionalization capabilities.
  • Thiolated oligonucleotides are crucial for surface modification and specific DNA targeting.
  • Colorimetric detection offers a visual method for identifying target molecules.

Purpose of the Study:

  • To synthesize and characterize silver/gold core-shell nanoparticles.
  • To functionalize these nanoparticles with thiolated oligonucleotides for DNA detection.
  • To develop a novel two-color assay for single-nucleotide polymorphism detection and validation.

Main Methods:

  • Synthesis of silver/gold core-shell nanoparticles.
  • Surface functionalization with thiolated oligonucleotides.
  • DNA detection assays using colorimetric changes.
  • Melting experiments to assess probe performance.

Main Results:

  • Silver/gold core-shell nanoparticles exhibit unique optical properties compared to pure gold nanoparticles.
  • Functionalized nanoparticles effectively detect target DNA.
  • Size-matched core-shell and gold nanoparticles show similar performance in DNA detection and melting.
  • Distinct optical signatures allow for differentiation between probes.

Conclusions:

  • Silver/gold core-shell nanoparticles are viable probes for DNA detection, offering distinct optical signatures.
  • The developed two-color method enables simultaneous detection and validation of single-nucleotide polymorphisms.
  • This approach expands the toolkit for precise genetic analysis and diagnostics.