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Updated: Jun 28, 2026

Optimization of Processing of Tiebangchui with Highland Barley Wine Based on the Box-Behnken Design Combined with the Entropy Method
Published on: May 19, 2023
Fluorimetric determination of histamine in wine and cider by using an anion-exchange column-FIA system and factorial
Gloria Del Campo1, Beatriz Gallego, Iñaki Berregi
1Applied Chemistry Department, Faculty of Chemistry, University of the Basque Country, Manuel Lardizabal 3, P.O. Box 1072, E-20018 San Sebastián, Spain.
Abstract:
A study has been performed of the conditions for the reaction of histamine with o-phthaldehyde in a flow injection analysis system employing three channels, using an anion-exchange column to eliminate sample matrix interferences. Factorial design was used to determine which operational parameters should be included in the optimization and their optimal values were found. The method developed shows good selectivity for histamine determination in alcoholic beverages. A linear response of up to 2.0mgl(-1) was observed and the detection and quantification limits were 30 and 101mugl(-1), respectively. The repeatability, measured by the R.S.D. for 10 replicate injections, was 0.84 and 0.52% for histamine solutions of 0.20 and 2.0mgl(-1), respectively. The recoveries obtained in wine and cider samples were close to 100% and a sample frequency of 24 samples per hour was achieved.
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