A clinical assessment of direct electrochemical urate measurements
Jodi S N Dutt1, Callum Livingstone, Marco F Cardosi
1Department of Chemistry, School of Biomedical and Natural Sciences, Nottingham Trent University, Nottingham, Notts NG11 8NS, UK.
Abstract:
The response characteristics of various carbon substrates towards the direct oxidative measurement of urate and other key purine biomarkers have been compared. A novel carbon fibre laminate assembly has been proposed as an alternative substrate for the preparation of disposable sensing strips. The fabrication method is generic and readily transferable to a number of sensor applications. Its performance in the determination of urate within biofluids (serum and plasma) has been critically assessed. An inter-laboratory pilot study demonstrated the bioanalytical efficacy of the approach with the responses validated through comparison with the standard colorimetric (uricase/peroxidase) assay.
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