Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photodegradation of expectorant drug ambroxol in seawater environment: Seasonal impact on a global scale.

Water research·2026
Same author

Unexpected widespread amyloid PET positivity in a patient with CADASIL.

Journal of neurology·2026
Same author

Early and severe masticatory muscle involvement in SOD1-ALS: a case report with biomarker-clinical dissociation.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·2026
Same author

A PbS quantum dot film as a hole transport layer for self-powered AgBiS<sub>2</sub> nanocrystal photodetectors.

Chemical communications (Cambridge, England)·2026
Same author

Artificial intelligence for detecting fetal orofacial clefts and advancing medical education.

Nature communications·2026
Same author

HER2∆16 directs luminal cell identity and estrogen receptor signaling in HER2+ breast cancer.

Nature communications·2026

Related Experiment Video

Updated: Jun 28, 2026

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation
11:18

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation

Published on: January 7, 2019

Conductometric nitrate biosensor based on methyl viologen/Nafion/nitrate reductase interdigitated electrodes.

Wang Xuejiang1, Sergei V Dzyadevych, Jean-Marc Chovelon

  • 1State Key Laboratory of Pollution Control and Resources Reuse, Tongji University, Shanghai, China.

Talanta
|October 31, 2008
PubMed
Summary

A novel conductometric enzyme biosensor offers a highly sensitive, fast, and stable method for detecting nitrate in water. This innovative sensor utilizes nitrate reductase (NR) for accurate and rapid water quality analysis.

More Related Videos

NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose
11:04

NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose

Published on: December 30, 2025

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Related Experiment Videos

Last Updated: Jun 28, 2026

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation
11:18

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation

Published on: January 7, 2019

NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose
11:04

NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose

Published on: December 30, 2025

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Area of Science:

  • Electrochemistry
  • Biosensor Technology
  • Environmental Science

Background:

  • Accurate nitrate determination in water is crucial for environmental monitoring and public health.
  • Existing methods for nitrate detection can be time-consuming or lack sensitivity.
  • Development of rapid, sensitive, and stable biosensors is needed for efficient water quality assessment.

Purpose of the Study:

  • To report the first highly sensitive, fast, and stable conductometric enzyme biosensor for nitrate determination in water.
  • To optimize fabrication and experimental parameters for enhanced biosensor performance.
  • To evaluate the operational and storage stability of the developed biosensor.

Main Methods:

  • Enzyme electrode modification using methyl viologen mediator and nitrate reductase (NR) from Aspergillus niger.
  • Cross-linking immobilization with glutaraldehyde, bovine serum albumin, and Nafion((R)) cation-exchange polymer.
  • Investigation of process parameters including pH, enzyme loading, and immobilization time.

Main Results:

  • The biosensor achieved 95% steady-state conductance in approximately 15 seconds.
  • A linear calibration range of 0.02–0.25 mM nitrate was established.
  • A low detection limit of 0.005 mM nitrate was achieved with a signal-to-noise ratio of 3.
  • The biosensor demonstrated good operational and storage stability over 2 weeks at 4°C.

Conclusions:

  • A novel conductometric enzyme biosensor provides a sensitive, rapid, and stable platform for nitrate detection in aqueous samples.
  • The optimized fabrication and immobilization techniques contribute to the biosensor's excellent performance characteristics.
  • This biosensor represents a significant advancement for real-time water quality monitoring.