A dual-wavelength resonance light scattering ratiometry of biopolymer by its electrostatic interaction with
Xiao Xia Dai1, Yuan Fang Li, Wei He
1College of Chemistry and Chemical Engineering, Key Laboratory of Eco-Environments of Three Gorges Reservoir Region under PRC National Education Ministry, Southwest University, Chongqing 400715, China.
Abstract:
A dual-wavelength resonance lighting scattering (DW-RLS) ratiometry is developed to detect anion biopolymer based on their bindings with cation surfactant. Using the interaction of Hyamine 1622 (HM) with fish sperm DNA (fsDNA) as an example, a dual-wavelength resonance light scattering (DW-RLS) ratiometric method of DNA was constructed. In Britton-Robinson buffer controlled medium, fish sperm DNA (fsDNA) could interact with Hyamine 1622 (HM), displaying significantly enhanced RLS signals. By measuring the RLS signals characterized at 300.0nm (I(300.0)) and the RLS intensity ratio (I(276.0)/I(294.0)), respectively, fsDNA over a wide dynamic range of content could be detected. Typically, when HM concentration is kept at 6.0x10(-5)moll(-1), using I(300.0) could detect fsDNA over the range of 50-2000ngml(-1) with the limit of 3.0ngml(-1), while using I(276.0)/I(294.0) could detect fsDNA over the range of 0.5-2500ngml(-1) with the limit of 0.05ngml(-1). Thus the latter so-called DW-RLS ratiometry is obviously superior to the former one. Based on the measurements of I(300.0) and I(276.0)/I(294.0) data, a Scatchard plot concerning the interaction between HM and fsDNA could be constructed and thus the binding number (n) and binding constant (K) could be available with the values of 13.5 and 1.35x10(5)mol(-1)l, and 11.9 and 1.65x10(5)mol(-1)l, respectively.
