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Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo
Published on: February 6, 2015
[Effect of chemotherapy with cisplatin and rapamycin against Hep-2 cells in vitro]
Wen-bin Lei1, Tao Jia, Zhen-zhong Su
1Institute of Otorhinolaryngology, Hospital of Otorhinolaryngology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China. leiwb2003@yahoo.com.cn
Objective:
To evaluate the effect of combined use of rapamycin and cisplatin in the chemotherapy of Hep-2 cells in vitro.
Methods:
The inhibitory effects of rapamycin and cisplatin, used alone or in combination, on the proliferation of Hep-2 cells were measured with MTT assay and median-effect plot analysis. The cell cycle changes after the treatment were analyzed using flow cytometry and Hoechst 33258 immunofluorescence staining.
Results:
The IC50 of rapamycin and cisplatin for inducing growth arrest of Hep-2 cells was 11.03 nmol/L and 8.81 micromol/L, respectively. Rapamycin alone caused cell cycle arrest of the Hep-2 cells in G1 phase. Rapamycin and cisplatin showed synergistic effects in the chemotherapy of Hep-2 cells (q > 1.15, King's Formula), causing significantly increased apoptosis ratio and growth inhibition rate of Hep-2 cells.
Conclusion:
Combined use of rapamycin and cisplatin significantly improves the chemotherapeutic effect against Hep-2 cells.
