Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Food Intake01:30

Regulation of Food Intake

Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...
Hormonal Regulation01:40

Hormonal Regulation

Hormones regulate a significant portion of digestion through activation of the neuroendocrine system. The neuroendocrine system of digestion contains many different hormones all with multiple functions that are both, directly and indirectly, involved in digestion.
Regulation of the Digestive System01:25

Regulation of the Digestive System

Digestive activity regulation hinges on three primary components. Activation is prompted by a multitude of mechanical and chemical indicators, primarily detected by receptors within the stomach and intestines' walls. These receptors predominantly respond to factors such as mechanical stretching of the organ walls, changes in pH and osmolarity, and the presence of digesting materials and their by-products.
The effectors in this regulation system are glands and smooth muscles. Activation of these...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Triggering and modulation of a complex behavior by a single peptidergic command neuron in <i>Drosophila</i>.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Neuropeptides from a praying mantis: what the loss of pyrokinins and tryptopyrokinins suggests about the endocrine functions of these peptides.

PeerJ·2025
Same author

Parvalbumin interneuron activity in autism underlies susceptibility to PTSD-like memory formation.

iScience·2024
Same author

Evolution of a Cockroach Allergen into the Major Protein of Termite Royal Jelly.

International journal of molecular sciences·2023
Same author

Pigment-dispersing factor is present in circadian clock neurons of pea aphids and may mediate photoperiodic signalling to insulin-producing cells.

Open biology·2023
Same author

Gut AstA mediates sleep deprivation-induced energy wasting in Drosophila.

Cell discovery·2023

Related Experiment Video

Updated: Jun 28, 2026

Studying the Activity of Neuropeptides and Other Regulators of the Excretory System in the Adult Mosquito
11:30

Studying the Activity of Neuropeptides and Other Regulators of the Excretory System in the Adult Mosquito

Published on: August 24, 2021

Regulatory peptides in fruit fly midgut.

Jan A Veenstra1, Hans-Jürgen Agricola, Azza Sellami

  • 1CNIC UMR 5228 CNRS, Université Bordeaux 1, 33400, Talence, France. j.veenstra@cnic.u-bordeaux1.fr

Cell and Tissue Research
|October 31, 2008
PubMed
Summary

This study maps regulatory peptides in the Drosophila melanogaster midgut, identifying endocrine cells producing allatostatins, neuropeptide F, diuretic hormone 31, and tachykinins. The research clarifies peptide expression in fruit fly gut endocrine cells.

More Related Videos

Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in Drosophila
06:49

Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in Drosophila

Published on: June 2, 2018

Physiological Experimentation with the Crayfish Hindgut: A Student Laboratory Exercise
10:07

Physiological Experimentation with the Crayfish Hindgut: A Student Laboratory Exercise

Published on: January 18, 2011

Related Experiment Videos

Last Updated: Jun 28, 2026

Studying the Activity of Neuropeptides and Other Regulators of the Excretory System in the Adult Mosquito
11:30

Studying the Activity of Neuropeptides and Other Regulators of the Excretory System in the Adult Mosquito

Published on: August 24, 2021

Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in Drosophila
06:49

Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in Drosophila

Published on: June 2, 2018

Physiological Experimentation with the Crayfish Hindgut: A Student Laboratory Exercise
10:07

Physiological Experimentation with the Crayfish Hindgut: A Student Laboratory Exercise

Published on: January 18, 2011

Area of Science:

  • Insect physiology
  • Neuroendocrinology
  • Gastrointestinal biology

Background:

  • The fruit fly Drosophila melanogaster midgut harbors diverse endocrine cells.
  • Regulatory peptides play crucial roles in insect gut function.
  • Previous studies suggested potential peptide production by insect midgut endocrine cells.

Purpose of the Study:

  • To immunolocalize and characterize regulatory peptides within the Drosophila melanogaster midgut.
  • To identify specific endocrine cell populations and their peptide products.
  • To clarify the expression of neuropeptide F (NPF) and related peptides in the adult fruit fly midgut.

Main Methods:

  • Immunohistochemistry using specific antisera against various regulatory peptides.
  • Localization of peptides within the anterior, middle, and posterior midgut sections.
  • Utilizing transgenic fly models to confirm peptide expression and cell types.

Main Results:

  • Identified endocrine cells producing allatostatins A, B, and C, NPF, diuretic hormone 31, and tachykinins.
  • NPF-producing endocrine cells were found in the anterior, middle, and initial posterior midgut, co-producing tachykinins.
  • Diuretic hormone 31 and tachykinins were localized in the caudal posterior midgut; tachykinins also occurred at midgut extremities.
  • Allatostatin A and C expression varied across midgut regions, with some cells producing both.
  • Small neuropeptide-F (sNPF) and pigment-dispersing factor were found in specific neural pathways.
  • Confirmed that adult Drosophila midgut endocrine cells exclusively produce NPF, not other suggested peptides like myosuppressin or sulfakinins.
  • Drosophila insulin gene Ilp3 was expressed in midgut muscle, not endocrine cells.

Conclusions:

  • The Drosophila melanogaster midgut possesses a complex array of endocrine cells producing distinct regulatory peptides.
  • NPF and tachykinins are co-expressed in specific midgut endocrine cell populations.
  • Allatostatin subtypes show regional specificity in their production.
  • This study provides a detailed map of peptide-producing endocrine cells in the fruit fly midgut, advancing our understanding of insect gut regulation.