Related Experiment Video
Updated: Jun 28, 2026

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
Published on: June 25, 2018
N-glycan trimming by glucosidase II is essential for Arabidopsis development
Pravina Soussilane1, Pravina Soussillane, Cecilia D'Alessio
1CNRS, UMR 6037, IFRMP 23, Bâtiment Biologie Extension, Faculté des Sciences, Mont-Saint-Aignan, France.
Abstract:
Glucosidase II, one of the early N-glycan processing enzymes and a major player in the glycoprotein folding quality control, has been described as a soluble heterodimer composed of alpha and beta subunits. Here we present the first characterization of a plant glucosidase II alpha subunit at the molecular level. Expression of the Arabidopsis alpha subunit restored N-glycan maturation capacity in Schizosaccharomyces pombe alpha- or alphabeta-deficient mutants, but with a lower efficiency in the last case. Inactivation of the alpha subunit in a temperature sensitive Arabidopsis mutant blocked N-glycan processing after a first trimming by glucosidase I and strongly affected seedling development.
Related Concept Videos
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Protein Folding Quality Check in the RER
Biosynthesis of Polysaccharides
Protein Glycosylation
Glycosylation occurs in...
Proteoglycans

