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Related Experiment Video

Updated: Jun 28, 2026

Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization
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Host muscle cell infiltration in cell-seeded plastic compressed collagen constructs.

Hamzeh Kayhanian1, Stephanie Jones, James Phillips

  • 1UCL Tissue Repair and Engineering Centre, Division of Surgical and Interventional Sciences, Institute of Orthopaedics and Musculoskeletal Sciences, Stanmore Campus, London, UK.

Journal of Tissue Engineering and Regenerative Medicine
|October 31, 2008
PubMed
Summary

Cell-seeded collagenous tissue constructs showed improved healing and mechanical properties in vivo. However, muscle infiltration was delayed, and nerve infiltration was absent after 5 weeks in a lapine model.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Plastic compression allows rapid production of collagenous tissue constructs.
  • Previous studies demonstrated benefits of cell-seeded constructs over acellular ones in vivo.
  • Cyclical tensile loading was applied to constructs implanted in a lapine intercostal space model.

Purpose of the Study:

  • To evaluate host muscle and nerve infiltration into cell-seeded and acellular collagenous tissue constructs.
  • To assess the timing and extent of myoblast and neuronal infiltration in vivo.
  • To compare infiltration in cell-seeded constructs versus acellular controls.

Main Methods:

  • Spiral acellular and cell-seeded constructs were implanted in a lapine model for 5 weeks.
  • Constructs were harvested and stained for desmin (myoblast marker) and neurofilament (neuronal marker).
  • Immunoperoxidase staining was used to visualize host cell infiltration.

Main Results:

  • Host muscle cells (myoblasts) began migrating into constructs by 3 weeks.
  • Muscle cell infiltration did not reach the core of the constructs by 5 weeks, indicating a delayed response.
  • No evidence of host nerve infiltration was observed in the constructs at 5 weeks.

Conclusions:

  • Cell-seeded collagenous constructs demonstrate delayed but present muscle infiltration.
  • The constructs lack significant innervation after 5 weeks of in vivo implantation.
  • Further optimization is needed to enhance vascularization, muscle, and nerve integration for improved functional tissue regeneration.