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Ribonucleosides as minor milk constituents.
E Schlimme1, K P Raezke, F G Ott
1Institute for Chemistry and Physics, Federal Dairy Research Centre, Kiel, FRG.
Summary
Milk ribonucleosides, including modified forms from RNA breakdown, exhibit stable levels throughout lactation. This stable pattern acts as a unique fingerprint for milk, aiding species and butter type differentiation.
Area of Science:
- Food Chemistry
- Biochemistry
- Analytical Chemistry
Background:
- Ribonucleosides are minor milk components with assumed species-specific patterns.
- Modified ribonucleosides, such as N1-methyladenosine and N6-carbamoylthreonyladenosine, originate from transfer RNA catabolism.
Purpose of the Study:
- To quantify ribonucleoside levels in milk throughout lactation.
- To assess the utility of ribonucleoside patterns for milk characterization and technological process analysis.
Main Methods:
- Quantification of unmodified and modified ribonucleosides in individual and bulk milk samples.
- Longitudinal study design across a whole lactation period.
- Analysis of ribonucleoside distribution during the churning process.
Main Results:
- Ribonucleoside levels, excluding the colostral phase, remained relatively constant throughout lactation.
- The identified ribonucleoside patterns were found to be stable and species-specific.
- Ribonucleoside profiles effectively differentiated between three German butter types.
Conclusions:
- Ribonucleosides are valuable markers for milk characterization across species and technological treatments.
- The 'fingerprint' provided by ribonucleoside patterns can be used to authenticate butter types.
- Minor milk constituents offer insights into milk composition and processing.