[Study on aqueous chemical constituents from the flower buds of Eugenia caryophylla]
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Hong-Yu Liu1, Shu Zhu, Katsuko Komatsu
1Center for Health Food Evaluation, State Food and Drug Administration, Beijing 100070, China. liuhynn@hotmail.com
Objective:
To study the aqueous chemical constituents of the flower buds of Eugenia caryophylla.
Methods:
The compounds were isolated by HP-20 resin column chromatography, RP-C18 column chromatography, preparative RP-C18 thin-layer chromatography, preparative RP-C18 high performance liquid chromatography, and their structures were established by NMR and MS evidences.
Results:
Five compounds were separated from the water extracts. Their structures were identified as quercetin-3-O-glucuronide (1), quercetin-3-O-glucuronide 6"-methyl ester (2), quercetin-3-O-glucopy-ranoside (3) , eugenyl-beta-rutinoside (4) and myricetin (5).
Conclusion:
Compounds 1-4 are isolated from the genus for the first time.
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