Related Experiment Video
Updated: Jun 28, 2026
Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System
Published on: October 16, 2013
Chemical engineering of mesenchymal stem cells to induce a cell rolling response
Debanjan Sarkar1, Praveen Kumar Vemula, Grace S L Teo
1Department of Medicine, Harvard-MIT Division of Heath Sciences and Technology, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA.
Abstract:
Covalently conjugated sialyl Lewis X (SLeX) on the mesenchymal stem cell (MSC) surface through a biotin-streptavidin bridge imparts leukocyte-like rolling characteristics without altering the cell phenotype and the multilineage differentiation potential. We demonstrate that the conjugation of SLeX on the MSC surface is stable, versatile, and induces a robust rolling response on P-selectin coated substrates. These results indicate the potential to increase the targeting efficiency of any cell type to specific tissue.
