Related Experiment Video
Updated: Jun 28, 2026
10:42
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
Published on: May 12, 2023
Seeing small molecules in action with bioorthogonal chemistry
Anuradha S Raghavan1, Howard C Hang
1The Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, 1230 York Avenue Box 250, New York, NY 10065, United States.
Drug Discovery Today
|November 1, 2008
Summary
Chemical probes and bioorthogonal chemistry accelerate drug discovery by enabling functional proteome characterization and precise target identification for novel small molecules. These advancements reveal new therapeutic targets and disease-related small molecules.
Area of Science:
- Chemical Biology
- Drug Discovery
- Proteomics
Background:
- Chemical probes are essential tools for drug discovery, targeting specific protein families.
- Small molecules with reactive groups and tags aid in functional proteome characterization.
- Determining inhibitor specificity across enzyme/protein classes is crucial.
Purpose of the Study:
- To review the application of bioorthogonal chemistries with chemical probes.
- To highlight opportunities for dissecting enzyme/protein family functions in vivo.
- To enable precise target identification for small molecules.
Main Methods:
- Utilizing chemical probes with bioorthogonal reactions.
- Functional proteome characterization.
- In vivo studies of enzyme/protein family functions.
Main Results:
- Unprecedented opportunities to dissect in vivo protein functions.
- Precise identification of small molecule targets.
- Enhanced characterization of small molecule inhibitors.
Conclusions:
- Advances in chemical probes and bioorthogonal reactions accelerate drug discovery.
- New therapeutic targets can be revealed.
- Facilitates discovery and characterization of disease-related small molecules.
More Related Videos
Related Concept Videos
03:31Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
01:19Protein Dynamics in Living Cells
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...