Related Experiment Video

Updated: Jun 28, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

MiR-433 and miR-127 arise from independent overlapping primary transcripts encoded by the miR-433-127 locus

Guisheng Song1, Li Wang

  • 1Departments of Medicine and Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA.

Plos One
|November 1, 2008
PubMed

Insights

Researchers identified the primary transcripts of microRNAs miR-433 and miR-127. These microRNA genes share overlapping coding regions, representing an economical gene structure in mammals.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are crucial regulators in cellular processes like development, metabolism, and carcinogenesis.
  • Limited knowledge exists regarding the primary transcripts and transcriptional regulation of miRNA genes.

Purpose of the Study:

  • To clone and characterize the primary transcripts of mouse miR-433 and miR-127.
  • To investigate the transcriptional regulation and gene structure of the miR-433-127 locus.

Main Methods:

  • Database mining for miRNA gene identification.
  • Experimental isolation of full-length primary miRNA transcripts.
  • Analysis of gene structure and expression patterns.

Main Results:

  • Successfully cloned two primary miRNAs (pri-miR-433 and pri-miR-127) from the miR-433-127 locus.
  • Demonstrated a 5'-3' unidirectional overlap between pri-miR-433 and pri-miR-127.
  • Revealed an economical gene structure with overlapping coding regions for these two miRNA genes.

Conclusions:

  • This study reports the novel, economical gene structure of miR-433 and miR-127.
  • This compact organization may represent a strategy for maximizing genetic information in mammals.
  • Provides foundational insights into miRNA gene regulation and structure.

More Related Videos

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...