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Updated: Jun 28, 2026

A Microfluidic Chip for ICPMS Sample Introduction

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

A micro circulating PCR chip using a suction-type membrane for fluidic transport.

Liang-Ju Chien¹, Jung-Hao Wang, Tsung-Min Hsieh

¹Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan.

Biomedical Microdevices

|November 1, 2008

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This study introduces a novel micromachined circulating polymerase chain reaction (PCR) chip with a microfluidic control module for rapid DNA amplification. The chip achieves high sample flow rates and excellent temperature uniformity, enabling efficient genetic identification and molecular diagnosis.

Area of Science:

Biotechnology
Microfluidics
Molecular Diagnostics

Background:

Conventional polymerase chain reaction (PCR) methods can be time-consuming and require complex equipment.
There is a need for rapid, portable, and efficient platforms for DNA amplification and genetic analysis.

Purpose of the Study:

To develop and characterize a novel micromachined circulating PCR chip.
To demonstrate the chip's capability for rapid DNA amplification and temperature control.

Main Methods:

A microfluidic control module with a suction-type membrane and microvalves was designed for liquid transportation.
Array-type microheaters and sensors were integrated for precise temperature control in open-type reaction chambers.
The chip's performance was validated by amplifying a hepatitis C virus gene.

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Main Results:

The microfluidic module achieved sample flow rates up to 18 microL/s.
Temperature uniformity within reaction chambers exceeded 90% with less than 1°C variation.
Successful amplification of hepatitis C virus DNA was demonstrated across a range of concentrations (10^5 to 10^2 copies/microL).

Conclusions:

The developed circulating PCR chip offers a promising platform for rapid genetic identification.
The chip's design facilitates efficient DNA amplification and molecular diagnostics.
This technology has potential applications in point-of-care diagnostics and genetic screening.