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Updated: Jun 28, 2026

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
Published on: May 10, 2020
[Effects of long-term fertilization on soil organic nitrogen components in paddy soil derived from red earth]
Hai-ying Zong1, Kai-rong Wang, Xiao-li Xie
1lnstitute of Agricultural Ecological and Environmental Health, Qingdao Agricultural University, Shandong, China. zheng18-981@163.com
Abstract:
A 16-year long-term fertilization experiment was conducted on paddy soil derived from red earth to investigate the effects of different fertilization patterns on the concentrations of soil organic nitrogen (N) components. When chemical fertilizers were applied only, the soil nitrogen content was slightly influenced. Organic fertilization, especially its combination with chemical fertilization, could significantly increase the contents of soil mineralizable N and organic nitrogen by 55.2% and 38.8%, respectively. In addition, organic fertilization could significantly improve the components of acid hydrolysable N, and lead to the increase of ammonium N (AN) , amino sugar N (ASN), and hydrolysable unidentified N (HUN) by 36.5%, 68.4%, and 73.9%, respectively. When the organic fertilization was combined with chemical fertilization, soil amino acid N content was increased by 71.1%, while HUN content was decreased by 34.5%. In all fertilization treatments, the cumulative amount of soil mineralized N increased with increasing incubation time. The content of soil mineralized N under organic fertilization and its combination with chemical fertilization was higher than that under chemical fertilization.
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