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Updated: Jun 28, 2026
Investigating von Willebrand Factor Pathophysiology Using a Flow Chamber Model of von Willebrand Factor-platelet String Formation
Published on: August 14, 2017
Effects of time of collection and sample processing on von Willebrand factor concentration in retired racing
L M Marín1, C G Couto, M C Iazbik
1Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH, USA. marin.25@osu.edu
Background:
Concentrations of von Willebrand factor (vWF) are less than 30% in approximately 10% of Greyhounds.
Hypothesis:
That sample collection, processing, and storage can affect the concentration of vWF in plasma of Greyhounds.
Animals:
Nineteen healthy former racing Greyhounds.
Methods:
Prospective study: Blood samples were obtained from the jugular vein of dogs at 4 times during the day. Samples were divided and processed in each of 3 ways. The 1st tube was centrifuged immediately, the 2nd tube was kept in a household refrigerator, and the 3rd tube was kept at room temperature for 3 hours before centrifugation and processing.
Results:
There were no significant differences in the vWF concentration between different sample storage and processing (P = 5.31). There was no statistically significant diurnal variation in vWF concentration in the samples evaluated (P = .37).
Conclusions And Clinical Importance:
Time of sample collection and short-term storage temperature do not influence the vWF concentration in retired racing Greyhounds.
