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Bone marrow stroma cells are susceptible to prion infection

Yuka Takakura1, Naohiro Yamaguchi, Takehiro Nakagaki

  • 1Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-12-4, Nagasaki 852-8523, Japan.

Biochemical and Biophysical Research Communications
|November 4, 2008
PubMed

Insights

Detecting abnormal prion protein (PrP-res) in bone marrow stromal cells (MSCs) offers a promising new diagnostic method for prion diseases like Creutzfeldt-Jakob disease (CJD). This technique could enable earlier and less invasive diagnosis.

Area of Science:

  • Neurology
  • Biochemistry
  • Cell Biology

Background:

  • Prion diseases are fatal neurodegenerative disorders.
  • Abnormal prion protein (PrP-res) is the sole biochemical marker for prion diseases.
  • Sensitive detection methods for PrP-res in biological samples are critically needed.

Purpose of the Study:

  • To investigate the potential of bone marrow stromal cells (MSCs) for PrP-res detection.
  • To evaluate ex vivo MSC expansion as a diagnostic tool for prion diseases.

Main Methods:

  • Primary cultured bone marrow stromal cells (MSCs) were used.
  • MSCs were infected with a mouse-adapted BSE strain to test PrP-res detection.
  • Bone marrow cells from Creutzfeldt-Jakob disease (CJD) patients were cultured and analyzed for PrP-res.

Main Results:

  • Cultured MSCs supported stable prion infection and expressed detectable PrP-res.
  • MSCs derived from bone marrow of CJD patients were positive for PrP-res.
  • PrP-res was successfully detected in expanded MSCs from CJD patients.

Conclusions:

  • Ex vivo expansion of MSCs can be used to detect PrP-res.
  • This method holds potential for earlier and less invasive diagnosis of prion diseases.
  • MSC-based PrP-res analysis may improve diagnostic capabilities for CJD and related disorders.

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