Related Experiment Video
Updated: Jun 28, 2026

Synthesis and Assay of Vibrio Quorum Sensing Inhibitors
Published on: May 31, 2024
Synthesis and ribonucleotide reductase inhibitory activity of thiosemicarbazones
Kesavan Krishnan1, Kumari Prathiba, Venkatesan Jayaprakash
1Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, Jharkhand 835 215, India.
Abstract:
Ribonucleotide reductase (RR) is an important therapeutic target for anticancer drugs. The structure of human RR features a 1:1 complex of two homodimeric subunits, hRRM1 and hRRM2. Prokaryotically expressed and highly purified recombinant human RR subunits, hRRM1 and hRRM2, were used for holoenzyme-based [(3)H]CDP reduction in vitro assay. Ten new thiosemicarbazones (7-16) were synthesized and screened for their RR inhibitory activity. Two thiosemicarbazones derived from p-hydroxy benzaldehyde (9 and 10) were found to be active but less potent than the standard, Hydroxyurea (HU). Guided by the activity of compounds 9 and 10, 11 new thiosemicarbazones (17-27) derived from p-hydroxy benzaldehyde were prepared and screened for their RR inhibitory activity. All the 11 compounds were more potent than HU.
Related Concept Videos
Inhibitors of Bacterial DNA Synthesis
Preparation and Reactions of Thiols
Preparation and Reactions of Sulfides
Inhibitors of Bacterial Protein Synthesis
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Inhibitors of Viral Protein Synthesis
