Dynamics of phosphorus and phytate-utilizing bacteria during aerobic degradation of dairy cattle dung

During organic wastes degradation, P is transformed which may affect its availability. In this study, the dynamics of P and the occurrence of phytate-utilizing bacteria (PUB) were evaluated during aerobic degradation of dairy cattle dung in laboratory-scale reactors for 105 d. The results showed an increase of water-soluble inorganic P (Pi) (from 570 to 1890 mg kg(-1)) and biomass P (from 390 to 870 mg kg(-1)) during the initial 40 d. After this period, water-soluble Pi remained constant (around 1500 mg kg(-1)) and biomass P decreased (around 220 mg kg(-1)) probably due to the decrease of easily available C in dung. Under the acidic conditions in the first 20 d there was an increase in concentration of Al (25 mg kg(-1)) and Fe (27 mg kg(-1)) ions. These ions were no longer detectable in the alkaline conditions occurring after 40 d. In the same period, the Ca concentration increased (from 1170 to 2370 mg kg(-1)) and chemical speciation revealed permanent association of Ca ions with Pi. Sequential P fractionation showed a decrease of organic P in NaHCO(3), NaOH and HCl fractions and an increase of residual P (25-52% with respect to total P). Analysis by (31)P NMR also showed a decrease (from 14% to 1.6%) of phytic acid content during final experimental period (60 and 105 d). The bacteriological analysis revealed various PUB involved in degradation of the dung. Two morphotypes, genetically characterized as Enterobacter and Rahnella, which were dominant under higher content of residual P, showed strong utilization of phytate in vitro.